Potential Attenuation of Cr(V1) in Hypoxic Water Using Fathead Minnow

Document Type

Grant Proposal

Date Accepted

Summer 2009

Project Description/Abstract

The molecular response to Cr(VI) is well understood in mammalian systems under normal physiological conditions, however, little is known about the effects of chromium in aquatic macro fauna. Further, chromium is an oxygen dependant oxy-anion. Therefore, the toxicity and bioavailability in fish may alter significantly when the dissolved oxygen (DO) in water plummets. I hypothesize the molecular toxicity caused by Cr(VI) will decrease when less oxygen is present. To evaluate aquatic ecosystems, I plan to assess changes in gene expression in the model freshwater organism, fathead minnow. Previous research has demonstrated that hypoxia delays the time of development. Therefore, I want to assess the teratogenic effects of larval development in fathead minnows exposed to chromium, hypoxia, or a combination of both stresses. Larvae will be monitored for mortality, spinal malformations, and decreased growth for 30 days exposures. RNA expression will analyze biomarkers of exposure and physiological effects using Q-PCR.

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