Significance of Steric Bulk in DNA Threading Intercalation Revealed through Force-Dependent Kinetics
DNA threading intercalation is a DNA binding mode, whereby a molecule must thread a bulky group through the DNA duplex to allow a planar central section to stack with the DNA base pairs. Molecules that bind to DNA via threading intercalation display incredibly slow association and dissociation kinetics. This is a result of the large duplex deformations required for the ligand to thread and unthread the DNA. While this binding mode has been investigated extensively, the specific molecular characteristics that affect the binding to DNA are still poorly understood.
Clark, A.G., Paramanathan, T., Westerlund, F., Lincoln, P., McCauley, M., Rouzina, I., & Williams, M. (2016). Significance of Steric Bulk in DNA Threading Intercalation Revealed through Force-Dependent Kinetics. Biophysical Journal 110(3, Supplement 1), 241a. https://doi.org/10.1016/j.bpj.2015.11.1328
Virtual Commons Citation
Clark, Andrew G.; Paramanathan, Thayaparan; Westerlund, Fredrik; Lincoln, Per; McCauley, Micah; Rouzina, Ioulia; and Williams, Mark (2016). Significance of Steric Bulk in DNA Threading Intercalation Revealed through Force-Dependent Kinetics. In Physics Faculty Publications. Paper 24.
Available at: http://vc.bridgew.edu/physics_fac/24