Title

Significance of Steric Bulk in DNA Threading Intercalation Revealed through Force-Dependent Kinetics

Publication Date

2016

Document Type

Abstract

Abstract

DNA threading intercalation is a DNA binding mode, whereby a molecule must thread a bulky group through the DNA duplex to allow a planar central section to stack with the DNA base pairs. Molecules that bind to DNA via threading intercalation display incredibly slow association and dissociation kinetics. This is a result of the large duplex deformations required for the ligand to thread and unthread the DNA. While this binding mode has been investigated extensively, the specific molecular characteristics that affect the binding to DNA are still poorly understood.

Original Citation

Clark, A.G., Paramanathan, T., Westerlund, F., Lincoln, P., McCauley, M., Rouzina, I., & Williams, M. (2016). Significance of Steric Bulk in DNA Threading Intercalation Revealed through Force-Dependent Kinetics. Biophysical Journal 110(3, Supplement 1), 241a. doi: 10.1016/j.bpj.2015.11.1328

Identifier

doi: 10.1016/j.bpj.2015.11.1328