Title

Removal of a High-fat Diet, but not Voluntary Exercise, Reverses Obesity and Diabetic-like Symptoms in Male C57BL/6J Mice

Publication Date

2017

Document Type

Article

Abstract

OBJECTIVE: Both the consumption of high-fat diets and exercise are known to produce alterations in metabolism and behavior. This study focuses on the effects of a change to a low-fat diet from a high-fat diet and voluntary exercise on obesity, type-2 diabetic-like symptoms, and locomotor behavior in male C57BL/6J mice.

DESIGN: Mice were initially given either a high-fat diet or regular chow, along with a cage with a running-wheel to mimic exercise, or one without, to determine to what extend exercise affects these symptoms. Then half of the mice given a high-fat diet were switched to regular chow to ascertain if the switch in diet would improve type-2 diabetic-like and obesity symptoms.

RESULTS: Wheel-running alone produced an improvement in insulin in mice continuously fed a high-fat diet (p=0.006), but running-wheels did not produce any further improvements in mice with regular chow replacement (p=0.999) or in controls (p=0.996). Replacement of a high-fat diet with regular chow led to physiological improvements in insulin (p=0.012) and leptin (p <0.001), glucose tolerance (p <0.001), and obesity (p <0.001), more so than exercise alone. Mice consuming a high-fat diet without a wheel exhibited reduced home-cage activity compared to controls after the diet switch (p=0.030), while no reduction was found in running-wheel activity between high-fat diet and regular chow consuming mice after switching diets (p=0.516).

CONCLUSIONS: These results suggest that exercise is only partially beneficial to improving health outcomes in mice consuming a high-fat diet, whereas incorporating a better diet, even without exercise, improves quality of health and can suppress T2DM symptoms and related conditions more so than exercise alone.

Original Citation

Hatzidis, A., Hicks, J.A., Gelineau, R.R., Arruda, N.L., Monteiro De Pina, I., O’Connell, K.E., & Seggio, J.A. (2017). Removal of a High-fat Diet, but not Voluntary Exercise, Reverses Obesity and Diabetic-like Symptoms in Male C57BL/6J Mice. HORMONES 16(1), 62-74. doi: 10.14310/horm.2002.1720

Identifier

doi: 10.14310/horm.2002.1720